The Isolation and Identification of Anthocyanin-Related GSTs in Chrysanthemum

Anthocyanin is the crucial pigment for coloration of red chrysanthemum flowers, which synthesizes in cytosol and transported to vacuole stable storage. In general, glutathione S-transferases (GSTs) play a vital role this transport. To date, there no functional GST reported chrysanthemums. Here, total 94 CmGSTs were isolated from genome, with phylogenetic analysis suggesting that 16 members them clustered into Phi subgroup was related anthocyanin Among them, expression CmGST1 positively correlated accumulation. Protein sequence alignment revealed included anthocyanin-related GST-specific amino acid residues. Further transient overexpression experiments tobacco leaves showed could promote addition, dual-luciferase assay demonstrated be regulated by CmMYB6, CmbHLH2 CmMYB#7, biosynthesis. Taken together, we suggested played key transport accumulation